#3 The promo with the rabies shots
Ambrose recalled an incident while talking with Jericho, which saw a writer explain to him that he would be getting rabies shots in a promo to innoculate himself against the fans, garnering him heel heat. He told the writer that he hated the promo. Later, Vince learned that Ambrose didn't want to do the promo. He met him and told him that the promo was good and would help him get tons of heel heat.
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The segment was another black mark on Ambrose's waning WWE career, and didn't help his fledgling heel turn one bit. According to Ambrose, he was being fed goofy promos like this for a while at that point and he had become tired of it. Eventually, the heel turn flopped and he was turned babyface during his final months in WWE.
Safe to say, with the benefit of hindsight, they should have just scrapped this when Ambrose complained.