· Atlanta United FC of Atlanta, Georgia
· Chicago Fire of Bridgeview, Illinois
· Columbus Crew SC of Columbus, Ohio
· D.C. United of Washington, D.C.
· *FC Cincinnati of Cincinnati, Ohio (2019)
· *Inter Miami CF Miami, Florida (2020)
· Montreal Impact of Montreal, Quebec
· *Nashville Nashville, Tennessee (2020)
· New England Revolution of Foxborough, Massachusetts
· New York City FC of New York City, New York
· New York Red Bulls of Harrison, New Jersey
· Orlando City SC of Orlando, Florida
· Philadelphia Union of Chester, Pennsylvania
· Toronto FC of Toronto, Ontario
*Teams that are to be added later
· Colorado Rapids of Commerce City, Colorado
· FC Dallas of Frisco, Texas
· Houston Dynamo of Houston, Texas
· LA Galaxy of Carson, California
· Los Angeles FC of Los Angeles, California
· Minnesota United FC of Minneapolis, Minnesota
· Portland Timbers of Portland, Oregon
· Real Salt Lake of Sandy, Utah
· San Jose Earthquakes of San Jose, California
· Seattle Sounders FC of Seattle, Washington
· Sporting Kansas City of Kansas City, Kansas
· Vancouver Whitecaps FC of Vancouver, British Columbia
